I have designed a homolog protein with sequence identity above 90 %? How can I measure protein stabilty and it ability to form aggreation. An example would be amyloid plage which aggregat in Alzheimer disease? Can I use dope score to measure protein stability or should I turn to rosetta desginer?
Knut J
On 11/26/2010 01:39 PM, Knut J Bjuland wrote: > I have designed a homolog protein with sequence identity above 90 %? How > can I measure protein stabilty and it ability to form aggreation. An > example would be amyloid plage which aggregat in Alzheimer disease? > Can I use dope score to measure protein stability or should I turn to > rosetta desginer?
The DOPE score will tell you how native-like your protein is (i.e. how "similar" it is to known crystal structures). So it won't necessarily tell you whether your protein is "stable".
Ben Webb, Modeller Caretaker
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