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Re: [modeller_usage] modeling with water, HETATM



On 01/10/2013 01:14 AM, Krupal Jethava wrote:
   I am trying to make model with Ligand (37 HetAtom) and 3 water
molecule. I used the same script as in manual but getting the error that
"Number of residue in alignment and pdb are different: 324      288 for
alignment entry: tseq.pdb"

We can't tell for sure without seeing your real input files, but by saying "FIRST:@:END ::@" (I think you mean "FIRST:@:END :@:") you are asking Modeller to read all residues from the first chain in the PDB file. So if the ligand and water residues are in a different chain, Modeller won't read them. (Rather than END:@, you could use the residue number and chain ID of the last ligand/water.) Note also that you'll need to set env.io.hetatm=True and env.io.water=True.

	Ben Webb, Modeller Caretaker
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