Have you tried it and didn't work?
This is how the alignment should look like. Just remember to superimpose the two templates (A, B) so that the loop ends look "joined".--On 7 May 2012 16:14, EGY <" target="_blank">> wrote:I searched the archives and could not find how to do what seems to be a simple procedure. I have a sequence that has high homology with a published X-ray structure A. A second state of the protein is defined by the conformation of a conserved loop. A related protein B containing the conserved loop is in the second state, albeit with lower homology. I would like to model the sequence in the second state using the structure A as a template, but using structure B to model the loop only. I believe I need an alignment like this:
proteinA aaaaaaaaaaaaaaaa-------------------aaaaaaaaaaaa
proteinB ----------------------------bbbbbbbbbbb---------------------
sequence cccccccccccccccccccccccccccccccccccccccccccccc
I realize this is a very simple question. I've been going through the manual and searching the archives and cannot figure out how to do it. Any help will be much appreciated.
Thanks.
EGY
_______________________________________________
modeller_usage mailing list
" target="_blank">
https://salilab.org/mailman/listinfo/modeller_usage
======================================================================
Thomas Evangelidis
PhD student
Biomedical Research Foundation, Academy of Athens
4 Soranou Ephessiou , 115 27 Athens, Greece
email: " target="_blank">
website: https://sites.google.com/site/thomasevangelidishomepage/
======================================================================
Thomas Evangelidis
PhD student
Biomedical Research Foundation, Academy of Athens
4 Soranou Ephessiou , 115 27
Athens, Greece
email: " target="_blank">
website:
https://sites.google.com/site/thomasevangelidishomepage/