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[modeller_usage] protein-ligand complex



Hi Modellers,

I would like to know, Is there anyway to modell a protein-ligand complex by modeller???????

If there is one, can you please tell me where can I get the information.

Thanking you.........

regards,
Praveen

On Mon, 21 Feb 2005 14:41:21 -0800
  wrote:
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Today's Topics:

  1. setting the DEVIATION (Ori Kalid)
  2. Atom Index is out of bounds: (Amjad Farooq)
  3. Re: setting the DEVIATION (Karsten Suhre)
  4. Regarding modelling (rajesh rengaraj)


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Message: 1
Date: Sun, 20 Feb 2005 16:18:35 +0200
From: "Ori Kalid" <>
Subject: [modeller_usage] setting the DEVIATION
To: <>
Message-ID: <00f801c51757$176c6320$551b4684@W2000EPRO>
Content-Type: text/plain; charset="windows-1255"

Hi Everyone,

Does anyone know how to control the XYZ randomization parameter DEVIATION? I tried modifying its value using a simple SET DEVIATION = 0.0, but nothing happens. The reasults are the same as for the default value of 4.0.

Thanks,

Ori

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Message: 2
Date: Mon, 21 Feb 2005 09:21:17 -0700
From: Amjad Farooq <>
Subject: [modeller_usage] Atom Index is out of bounds:
To: 
Message-ID:
	<>
Content-Type: TEXT/plain; CHARSET=US-ASCII

Dear Modeller users,

I am trying to model the structure of a 300-residue protein containing a phospho-threonine (PTH). After writing the topology of PTH in top_heavy.lib and specifying PTH in restype.lib under the modlib
subdirectory, I get this error upon running MODELLER:

csrrng__299E> Atom index is out of bounds: 2392 2391 recover____E> ERROR_STATUS >= STOP_ON_ERROR: 1 1

As you can see MODELLER does not complain about PTH but some general error. Has anyone encountered something like this before? How can this be fixed? Interestingly, when I substitute PTH with THR, MODELLER
generates no such error and models the structure nicely.

Any help will be greatly appreciated.

Many thanks,
Amjad

****************************************
AMJAD FAROOQ PhD
Assistant Professor

Dept of Biochemistry & Molecular Biology
Miller School of Medicine
University of Miami
Gautier Building, Rm 214
1011 NW 15th Street
Miami, FL 33136

tel 305-243-2429
fax 305-243-3955

http://farooqlab.ws
****************************************



------------------------------

Message: 3
Date: Mon, 21 Feb 2005 09:45:37 +0100
From: Karsten Suhre <>
Subject: Re: [modeller_usage] setting the DEVIATION
To: "Ori Kalid" <>, <> Message-ID: <>
Content-Type: text/plain;  charset="windows-1255"


Hi Ori,

Does anyone know how to control the XYZ randomization parameter DEVIATION? I tried modifying its value using a simple SET DEVIATION = 0.0, but nothing happens. The reasults are the same as for the default value of 4.0.

Try to generate more than one models in one go by using the following commands:

SET STARTING_MODEL= 1 # index of the first model SET ENDING_MODEL = 10 # index of the last model

Also, I understand that the initial random seed has to be a negative value in order to produce different models in different runs, e.g. use

SET RAND_SEED     = -42

I hope this helps,

Kind regards, Karsten.

PS: Don't set DEVIATION to zero if you want different models.

PPS: You can also try different MD_LEVEL parameters to get different models (of course the models will be of different quality, but in some applications, i.e. for molecular replacement, this may be a good idea)

SET MD_LEVEL = 'refine_3'


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Message: 4
Date: Mon, 21 Feb 2005 09:05:26 +0000 (GMT)
From: rajesh rengaraj <>
Subject: [modeller_usage] Regarding modelling
To: 
Message-ID: <>
Content-Type: text/plain; charset="iso-8859-1"

Hello modellers,

Is it possible to model 1249 amino acids at a stretch by using modeller, where the target sequence is having less similarty with the template.

I found the template by using the fold recognition method.

       Please help me modellers.

		
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End of modeller_usage Digest, Vol 4, Issue 19
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