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[modeller_usage] CHECK_ALIGNMENT accepts it, but ROUTINE = 'model' does not.



Hi,

I'm learning how to use modeller (release 6 with linux red hat 9).
While CHECK_ALIGNMENT seems accept my alignment, calling the
routine 'model' gives me an error when trying to read that same
alignment.

I 'm trying to model 41 residues into the first chain of the PDB file
1GL2.pdb (after this 'practice run' I will try to model the four chains of the
structure at the same time).

Following are relevant parts of scripts+logs.
Any ideas will be greatly appreciated!!!

Javier.

modeller.alignment.ali
----------------------
>P1;1GL2
structureX:1GL2:20:A:60:A:Endobrevin:rattus norvegicus:1.9:0.1370
EGVKNIMTQNVERILARGENLDHLRNKTEDLEATSEHFKTT
*

>P1;1GGX
sequence:1GGX:1: :41: :ykt6:rattus norvegicus::
DETKIILHNTMESLLERGEKLDDLVSKSEVLGTQSKAFYKT
*

chech_alignment.top
-------------------
SET OUTPUT_CONTROL = 1 1 1 1 1
SET ATOM_FILES_DIRECTORY = './../atom_files'

READ_ALIGNMENT FILE = 'modeller.alignment.ali', ALIGN_CODES = 'all'
WRITE_ALIGNMENT FILE = 'alignment.ali'
CHECK_ALIGNMENT
	

chech_alignment.log (last part of the file)
-------------------
openf5__224_> Open       11  OLD  SEQUENTIAL  modeller.alignment.ali

Read the alignment from file       : modeller.alignment.ali
Total number of alignment positions:    41

  #  Code        #_Res #_Segm PDB_code    Name

-------------------------------------------------------------------------------
  1     1GL2 A      41      1        1GL2 Endobrevin
  2       1GGX      41      1        1GGX ykt6
TOP_________>     4    4 WRITE_ALIGNMENT FILE = 'alignment.ali'

openf5__224_> Open       14  UNKNOWN  SEQUENTIAL  alignment.ali
TOP_________>     5    5 CHECK_ALIGNMENT

check_a_343_> >> BEGINNING OF COMMAND
openf5__224_> Open       11  OLD  SEQUENTIAL  ./../atom_files/1GL2.atm

check_ali___> Checking the sequence-structure alignment.

Implied target CA(i)-CA(i+1) distances longer than  8.0 angstroms:

ALN_POS  TMPL  RID1  RID2  NAM1  NAM2     DIST
----------------------------------------------
END OF TABLE
check_a_344_> << END OF COMMAND

Dynamically allocated memory at finish [B,kB,MB]:
3061621    2989.864     2.920
Starting time : 2004/06/08
16:10:06.379
Closing time : 2004/06/08
16:10:13.541
Total CPU time [seconds]                                 :       3.47


modelling.top
-------------
INCLUDE

SET OUTPUT_CONTROL = 1 1 1 1 1
SET ALNFILE  = './alignment.ali'
SET KNOWNS   = '1GL2'
SET SEQUENCE = '1GGX'
SET ATOM_FILES_DIRECTORY = '../atom_files'
SET STARTING_MODEL= 1
SET ENDING_MODEL  = 1


CALL ROUTINE = 'model'


modelling.log (last part of the file)
-------------
TOP_________>   111  711 CALL ROUTINE = 'model'

TOP_________>   112  399 CALL ROUTINE = 'getnames'

TOP_________> 113 509 STRING_IF STRING_ARGUMENTS = MODEL 'undefined', OPERATION; = 'EQ', THEN = 'STRING_OPERATE OPERATION = CONCATENA; TE, STRING_ARGUMENTS = SEQUENCE .ini, RESULT = MODEL'

TOP_________> 114 510 STRING_IF STRING_ARGUMENTS = CSRFILE 'undefined', OPERATI; ON = 'EQ', THEN = 'STRING_OPERATE OPERATION = CONCATE; NATE, STRING_ARGUMENTS = SEQUENCE .rsr, RESULT = CSRFILE;
                     '

TOP_________> 115 511 STRING_OPERATE OPERATION = 'CONCATENATE', ; STRING_ARGUMENTS = SEQUENCE '.sch', RESULT = SCHFILE

TOP_________> 116 512 STRING_OPERATE OPERATION = 'CONCATENATE', ; STRING_ARGUMENTS = SEQUENCE '.mat', RESULT = MATRIX_FI;
                     LE

TOP_________>   117  513 SET ROOT_NAME = SEQUENCE

TOP_________>   118  514 RETURN

TOP_________>   119  400 CALL ROUTINE = 'homcsr'

TOP_________> 120 106 READ_ALIGNMENT FILE = ALNFILE, ALIGN_CODES = KNOWNS SEQUE;
                     NCE


Dynamically allocated memory at amaxseq [B,kB,MB]: 2205269 2153.583 2.103
openf5__224_> Open       11  OLD  SEQUENTIAL  ./alignment.ali

Dynamically allocated memory at amaxbnd [B,kB,MB]: 3061621 2989.864 2.920
openf5__224_> Open       11  OLD  SEQUENTIAL  ./alignment.ali
read_al_375E> Unknown residue type,position,sequence: 1 1
recover____E> ERROR_STATUS >= STOP_ON_ERROR:        1       1

Dynamically allocated memory at finish [B,kB,MB]: 3061621 2989.864 2.920 Starting time : 2004/06/08 16:07:55.650 Closing time : 2004/06/08 16:08:04.684
Total CPU time [seconds]                                 :       4.30


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