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Re: [modeller_usage] structure not read in



Dear Sir,
It gives me the following message:

Dynamically allocated memory at         amaxseq [B,kB,MB]:      2205269   
2153.
583     2.103
openf5__224_> Open       11  OLD  SEQUENTIAL  align.ali
rdpir___376W> Not enough fields in the second line of the sequence entry: 
struc
tureX:1piea:9    : :396  : :galactokinase:Lactococcus lactis: 2.10
              Actual fields, should be:        9       10
              (Fields are separated by a colon, :)
rdpir___376W> Not enough fields in the second line of the sequence entry: 
seque
nce:galk:1    : :392   : :galk:Homo sapiens
              Actual fields, should be:        8       10
              (Fields are separated by a colon, :)

Dynamically allocated memory at         amaxbnd [B,kB,MB]:      8050641   
7861.
954     7.678
openf5__224_> Open       11  OLD  SEQUENTIAL  align.ali
rdpir___376W> Not enough fields in the second line of the sequence entry: 
struc
tureX:1piea:9    : :396  : :galactokinase:Lactococcus lactis: 2.10
              Actual fields, should be:        9       10
              (Fields are separated by a colon, :)
rdpir___376W> Not enough fields in the second line of the sequence entry: 
seque
nce:galk:1    : :392   : :galk:Homo sapiens
              Actual fields, should be:        8       10
              (Fields are separated by a colon, :)

Read the alignment from file       : align.ali
Total number of alignment positions:   402

  #  Code        #_Res #_Segm PDB_code    Name
-------------------------------------------------------------------------------
  1      1piea     388      1       1piea galactokinase
  2       galk     392      1        galk galk
TOP_________>   121  107 CHECK_ALIGNMENT

check_a_343_> >> BEGINNING OF COMMAND
pdbnam__217W> Filename for PDB code not found:  1piea
              Directories:  ./
              Extensions :   :.atm:.pdb:.ent:.crd
rdabrk__288W> Protein not accepted:        1
check_a_337E> Structure not read in:        1
recover____E> ERROR_STATUS >= STOP_ON_ERROR:        1       1

Dynamically allocated memory at          finish [B,kB,MB]:      8050641   
7861.
954     7.678
Starting time                                            : 2004/01/13 
11:55:59.
546
Closing time                                             : 2004/01/13 
11:56:00.
718


Please guide me.

Thanking you,

> On Sun, Jan 11, 2004 at 10:20:34AM +0530, Jaimin Shah wrote:
>> I am a novice for this program. I would really appreciate it if you can
>> expedite matters for me by solving my hurdles.
>> I am getting the message:
>> check_a_343_> >> BEGINNING OF COMMAND
>> check_a_337E> Structure not read in:  1     1
>
> Perhaps you have a sequence mismatch between your structure and your
> alignment? It's hard to tell from the output you've posted here. What
> errors or warnings do you get prior to that? I suggest you also put
> SET OUTPUT_CONTROL = 1 1 1 1 1
> at the start of your TOP file, if you haven't already, to increase the
> amount of Modeller output.
>
> 	Ben Webb, Modeller Caretaker
> --
>              http://www.salilab.org/modeller/
> Modeller mailing list: http://salilab.org/mailman/listinfo/modeller_usage
>


Jaimin Shah
M.Sc. (Biotechnology)
I.I.T. Powai