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RE: Problem with Sequence_Search



Hi, 

It's really hard to guess what's happening without looking at detailed
.log file, .top file, etc. If you could post those at the list it would
be helpful.

Thanks,
Bozidar


-----Original Message-----
From: 
[">mailto:] On Behalf Of
Thorsten Schweikardt
Sent: Friday, March 01, 2002 10:54 AM
To: 
Subject: Problem with Sequence_Search



Hi Modellers,

I am having a problem with the 'sequence_search' command
in Modeller6, Modeller crashes after ~5 hours of searching 
with the following message (which is always the same, and 
looks similar to the one Francesco Musiani reported yesterday):

projekte/limpo> mod6v1 search.top
/home/thorsten/modeller6v1/bin/mod6v1: line 41:  1774 Killed      $NICE
${EXECUTABLE} $STEERF
>$LOGF                      
I have already replaced the CHAINS files with the files from the new
distribution, so the 1xnb-error does not occur anymore. As advised, I
checked the stacksize, it is set to unlimited in the .cshrc-file, and
remains set to this value before and after modeller's crash. I also
tried different sequence-files, 
but there was no effect.

Modeller is running on a Pentium3-800, SUSE-Linux 7.1, 640 MB RAM.

Can anyone tell me what this error message means and
how to solve this problem?

Thanks in advance!

Thorsten Schweikardt
Molecular Biophysics
University of Mainz
Germany




My files:

search.top:
-----------

SET OUTPUT_CONTROL = 1 1 1 1 0
SET FILE = 'limpo.pir'
SEQUENCE_SEARCH ALIGN_CODES = 'limpo', DATA_FILE  = ON


limpo.pir:
----------

>P1;limpo
sequence:limpo:::::::0.00:0.00
TLHDKQIRICHLFEQLSSATVIGDGDKHKHSDRLKNVGKLQPGAIFSCFHPDHLEEARHL
YEVFWEAGDFNDFIEIAKEARTFVNEGLFAFAAEVAVLHRDDCKGLYVPPVQEIFPDKFI
PSAAINEAFKKAHVRPEFDESPILVDVQDTGNILDPEYRLAYYREDVGINAHHWHWHLVY
PSTWNPKYFGKKKDRKGELFYYMHQQMCARYDCERLSNGMHRMLPFNNFDEPLAGYAPHL
THVASGKYYSPRPDGLKLRDLGDIEISEMVRMRERILDSIHLGYVISEDGSHKTLDELHG
TDILGALVESSYESVNHEYYGNLHNWGHVTMARIHDPDGRFHEEPGVMSDTSTSLRDPIF
YNWHRFIDNIFHEYKNTLKPYDHDVLNFPDIQVQDVTLHARVDNVVHTFMREQELELKHG
INPGNARSIKARYYHLDHEPFSYAVNVQNNSASDKHATVRIFLAPKYDELGNEIKADELR
RTAIELDKFKTDLHPGKNTVVRHSLDSSVTLSHQPTFEDLLHGVGLNEHKSEYCSCGWPS
HLLVPKGNIKGMEYHLFVMLTDWDKDKVDGSESVACVDAVSYCGARDHKYPDKKPMGFPF
DRPIHTEHISDFLTNNMFIKDIKIKFHE*



logfile:
--------


                         MODELLER 6v1, 17 Jan 2002

     PROTEIN STRUCTURE MODELLING BY SATISFACTION OF SPATIAL RESTRAINTS

                                                                 
                     Copyright(c) 1989-2002 Andrej Sali          
                            All Rights Reserved                  
                                                                 
                            Written by A. Sali                   
          with help from A. Fiser, R. Sanchez, M.A. Marti-Renom, 
                   B. Jerkovic, A. Badretdinov, F. Melo,         
                       J.P. Overington & E. Feyfant              
                   Rockefeller University, New York, USA         
                     Harvard University, Cambridge, USA          
                 Imperial Cancer Research Fund, London, UK       
             Birkbeck College, University of London, London, UK  


Kind, OS, HostName, Kernel, Processor: 4, Linux at2 2.4.0-4GB i686
Date and time of compilation         : 01/24/2002 16:28:15
Job starting time (YY/MM/DD HH:MM:SS): 2002/03/01  11:51:45.460

TOP_________>     2    2 SET FILE = 'limpo.pir' 
 
TOP_________>     3    3 SEQUENCE_SEARCH ALIGN_CODES = 'limpo',
DATA_FILE  = ON 
 
openf5__224_> Open       11  OLD  SEQUENTIAL
${MODINSTALL6v1}/modlib//CHAINS_3.0_40_XN.cod
openf5__224_> Open       11  OLD  SEQUENTIAL
${MODINSTALL6v1}/modlib//CHAINS_3.0_40_XN.cod
openf5__224_> Open       11  OLD  SEQUENTIAL
${MODINSTALL6v1}/modlib//CHAINS_all.seq
openf5__224_> Open       11  OLD  SEQUENTIAL
${MODINSTALL6v1}/modlib//CHAINS_all.seq
openf5__224_> Open       11  OLD  SEQUENTIAL
${MODINSTALL6v1}/modlib//as1.sim.mat
rdrrwgh_268_> Number of residue types:       20
openf5__224_> Open       11  OLD  SEQUENTIAL  limpo.pir


seqsearc_>    1  limpo  1xnb    628  185  104   16.56   56.22  135506.30

 _aln.pos         10        20        30        40        50        60
limpo
TLHDKQIRICHLFEQLSSATVIGDGDKHKHSDRLKNVGKLQPGAIFSCFHPDHLEEARHLYEVFWEAG 
1xnb
ASTD-------YW-Q--NWT---DG------------G----G-I-----------------V----- 
 _consrvd    *          *    *   **            *    * *
*


 _aln.p   70        80        90       100       110       120       130
limpo
DFNDFIEIAKEARTFVNEGLFAFAAEVAVLHRDDCKGLYVPPVQEIFPDKFIPSAAINEAFKKAHVRP 
1xnb
--N-----A------VN-G--S-G------------GNY--------------S--VN--W------- 
 _consrvd   *     *      ** *                 * *              *   *


 _aln.pos  140       150       160       170       180       190
200
limpo
EFDESPILVDVQDTGNILDPEYRLAYYREDVGINAHHWHWHLVYPSTWNPKYFGKKKDRKGELFYYMH 
1xnb
----S-------NTGN-----F-V------VG-KG--W-------TTGSP--F-----RT--I-NY-N 
 _consrvd     *        ***              **     *        *  *  *     *
*


 _aln.pos    210       220       230       240       250       260
270
limpo
QQMCARYDCERLSNGMHRMLPFNNFDEPLAGYAPHLTHVASGKYYSPRPDGLKLRDLGDIEISEMVRM 
1xnb
----A---------GV--WAP-NG-N----GY---LT-L----Y------GWT-R-------SPLI-- 
 _consrvd     *         *     * *       **   **      *      *   *
*


[....]

The alignments stop with 1hv8.pdb, before the summary of the
1hv8-alignment 
is written.